p13 Myeloid Marker Mouse Monoclonal Antibody [Clone ID: BM-3]

CAT#: AM32300PU-N

p13 Myeloid Marker mouse monoclonal antibody, clone BM-3, Purified

Specifications

Product Data

• Clone Name: BM-3
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution:

  ELISA: 1/5,000-1/20,000.
  Western Blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections: (1/100).
  The antibody does not stain tissue after paraffin treatment.

• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated Human peripheral blood lymphocytes.
• Specificity: This purified Mouse monoclonal antibody reacts specifically with p13 in Human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues.
  The antibody recognizes a 13 kDa band corresponding to p13.
  Cross reactivity from other sources has not been determined.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: One year from despatch.

• Background:

  p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues. BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13,000 Dalton protein.