Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: M9]

CAT#: AM32291SU-N

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone M9, Supernatant

Specifications

Product Data

• Clone Name: M9
• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections: ~1/150, preferably in PBS.
  Immunohistochemistry on Paraffin Sections: A positive result on paraffin embedded tissues could be obtained with a TUF pretreatment at a antibody dilution of 1/10.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytokeratin isolated from Human breast carcinoma cell line MCF-7.
• Specificity:

  In Immunoblotting experiments the antibody clone M9 recognizes only CytoKeratin 18 (45 kD) and lacks cross reactivity with other Cytokeratins.
  The antibody stains especially simple epithelia.
  Cross reactivity
  GFAP : not detectable
  Neurofilament : not detectable
  Vimentin : not detectable
  Desmin : not detectable

• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Preservative: Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Gene Name: keratin 18
• Database Link:
  Entrez Gene 3875 Human
  UniProt ID P05783
• Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18