CD21 (CR2) Mouse Monoclonal Antibody [Clone ID: 2G9]

CAT#: AM32268SU-N

CD21 (CR2) mouse monoclonal antibody, clone 2G9, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human Tonsil stained with CD21 antibody clone 2G9

Specifications

Product Data

• Clone Name: 2G9
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Sections.
  Recommended Dilutions: 1/25-1/100
  Recommended Positive Control: Tonsil, Lymph Node.
  Preparation and Pretreatment
  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity:

  This antibody recognises the Human CD21 cell surface antigen, a 140 glycoprotein. CD21 is expressed by mature B lymphocytes.
  Anti-CD21 is useful in the identification of follicular dendriditic cell matrix found in normal lymph nodes and tonsillar tissue.
  This antibody also labels follicular dendritic cell tumor/sarcomas. The antigen is absent on T-lymphocytes, monocytes, and granulocytes.

• Formulation: Tris Buffer, pH 7.3-7.7
  State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Stabilizer: 0.9% BSA
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement component 3d receptor 2
• Database Link:
  Entrez Gene 1380 Human
  UniProt ID P20023
• Background: CD21, also known as CR2, C3d receptor and EBV receptor, is expressed strongly on mature B cells, follicular dentritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
• Synonyms: Complement receptor type 2, CR2, C3DR, C3d receptor, EBV Receptor, Dendritic Cell Marker