CD247 Rabbit Monoclonal Antibody [Clone ID: MRQ-39]
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Specifications
Product Data | |
Clone Name | MRQ-39 |
Applications | IHC |
Recommended Dilution | Immunohistochemistry on Frozen and Paraffin Sections: 1/100-1/500. Instructions for use: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil, T-Cell lymphomas. Staining Pattern: Membranous. |
Reactivities | Human |
Host | Rabbit |
Isotype | IgG1 |
Clonality | Monoclonal |
Specificity | Anti-CD3 has been considered the best all around T-cell marker. This antibody reacts with an antigen present in early thymocytes. The positive staining of this marker may represent a sign of early commitment to the T-Cell lineage. |
Formulation | PBS, pH 7.4 State: Supernatant State: Liquid tissue culture supernatant Stabilizer: 0.9% BSA Preservative: 0.09% Sodium Azide |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Gene Name | CD247 molecule |
Database Link | |
Synonyms | T3Z, TCRZ, CD3 zeta chain |
Reference Data |
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