PAI1 (SERPINE1) Mouse Monoclonal Antibody [Clone ID: MA-33H1F7]

CAT#: AM32111PU-N

PAI1 (SERPINE1) mouse monoclonal antibody, clone MA-33H1F7, Liquid

Specifications

Product Data

• Clone Name: MA-33H1F7
• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Western blotting.
  Immuno Assays.
  Immunohistochemistry on Frozen Sections.
  Inhibition: For inhibition of biological activity dilutions have to be made according to the amounts PAI-1 to be inactivated.
• Reactivities: Human, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity:

  The epitope of Monoclonal antibody MA-33H1F7 is predominantly composed of three residues (Lys¹⁵⁴/Glu¹³⁰/Arg¹³¹), positioned virtually linearly in the three-dimensional structure.
  The epitope of the antibody does not cover the complete alpha-helix F and turn connecting alpha-helix F and beta-strand s3A, but is restricted to the hinge region between alpha-helix F and the main part of the PAI-1 molecule.
  The Monoclonal antibody MA-33H1F7 is a switching antibody, capable of inducing a non-inhibitory substrate form of PAI-1.

• Formulation: PBS
  State: Liquid
  State: Liquid 0.2 µm filtered
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: serpin family E member 1
• Database Link:
  Entrez Gene 18787 MouseEntrez Gene 24617 RatEntrez Gene 5054 Human
  UniProt ID P05121
• Background: Plasminogen activator inhibitor type-1 (PAI-1), a member of the serine protease inhibitor (serpin) superfamily is an important protein in the regulation of fibrinolysis. PAI-1 is unique among the serpins because of its functional and conformational flexibility. PAI-1 is the most important physiological inhibitor of both tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). Increased PAI-1 levels are associated with thrombotic events and is an established risk factor for cardiovascular diseases. The active conformation PAI-1 inhibits its target proteinases by the formation of a stable, inactive complex. Although PAI-1 is synthesized as an active molecule, it converts spontaneously to an inactive, latent form that can be partially reactivated by denaturing agents. In addition, a third conformation reacting as a non-inhibitory substrate towards various target proteinases has been identified.
• Synonyms: Serpin E1, PLANH1, Plasminogen Activator Inhibitor 1, PAI-1, PAI

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, p53 signaling pathway