Fibrinogen (citrullinated) Mouse Monoclonal Antibody [Clone ID: 20B2]

CAT#: AM32003PU-N

Fibrinogen (citrullinated) mouse monoclonal antibody, clone 20B2, Purified

Product Images

Figure 1: Specificity of Anti-Deiminated Murine Fibrinogen Immunoglobulin, clone 20B2, determined by ELISA. Antibody fraction (0.5 mg/ml) 2000X diluted in PBS containing 0.05% tween-20 and 5% non fat dry milk. Antibody was tested on both rabbit PAD2 deimi

Specifications

Product Data

• Clone Name: 20B2
• Applications: ELISA
• Recommended Dilution: ELISA.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Deiminated Murine Fibrinogen peptide.
• Specificity:

  Specificity has been tested in ELISA (Figure 1).
  Crossreacts with deiminated Human Fibrinogen.
  Additional tests for cross reactivity have not yet been performed.

• Formulation: 10mM Ammonium Bicarbonate buffer
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in 200µl.
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C .
  Add 0.05% Sodium Azide for longer storage.
  
• Stability: Shelf life: one year from despatch.
• Background: Fibrinogen is a protein produced by the liver which helps stop bleeding by helping blood clots to form. Fibrinogen gets deiminated (conversion from arginin to citrullin) by Peptidyl Arginine Deïminase (PAD) in inflamed joints in patients that develop rheumatoid arthritis.Citrulline, while being an amino acid, is not built into proteins during protein synthesis, as it is not coded for by DNA, yet several proteins are known to contain citrulline. These citrulline residues are generated by a family of enzymes called peptidylarginine deiminases (PADs), which convert arginine into citrulline in a process called citrullination or deimination. Proteins that normally contain citrulline residues include myelin basic protein (MBP), filaggrin, and several histone proteins, while other proteins, like fibrin and vimentin can get citrullinated during cell death and tissue inflammation. Patients with rheumatoid arthritis often (at least 80% of them) develop an immune response against proteins containing citrulline. Although the origin of this immune response is not known, detection of antibodies reactive with citrulline containing proteins or peptides is now becoming an important help in the diagnosis of rheumatoid arthritis.
• Synonyms: FGA, FGB, FGG
• Note:

  Recommended Solvent: 100 mM PBS or Tris-HCl, pH 7.0–8.0
  For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
  NOTE: Be careful opening the vial since the antibody resides in a vacuum.