C5R1 (C5AR1) Rat Monoclonal Antibody [Clone ID: 8D6]

CAT#: AM31865FC-N

C5R1 (C5AR1) rat monoclonal antibody, clone 8D6, FITC

Specifications

Product Data

• Clone Name: 8D6
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Immunohistochemistry on frozen sections.
  Rezeptor blockage assays (unpublished results).
  
  Tissue Distribution by Flow Cytometry Analysis:
  Cell Source: Human PBL
  Cell Concentration: 1x10e6 cells per test
  Antibody Concentration Used: 1.0 μg/10e6 cells
  Secondary Antibody: Streptavidin FITC 1/500 dilution
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: RBL-2H3 (rat basophilic leukemia) transfected cells expressing human C5aR on the cell surface
  Donor: Lou/c rat spleen cells
• Specificity: This monoclonal antibody detects an epitope on the N terminus (aa 1-15) of the human C5a receptor (also known as CD88) found on human myeloid cells including granulocytes and monocytes/macrophages. It does not bind to tubular epithelial cells in situ under normal or inflammatory conditions. C5aR binds to C5a, a 74 aa peptide cleaved from complement protein C5. C5a receptor has seven transmembrane segments and is a member of the rhodopsin subfamily of G protein coupled receptors.
• Formulation: PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Fluorescein isothiocyanate isomer 1
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement component 5a receptor 1
• Database Link:
  Entrez Gene 728 Human
  UniProt ID P21730
• Synonyms: C5a-R, C5aR, C5AR1, Complement Component 5a Receptor 1

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Neuroactive ligand-receptor interaction