Ly-76 / TER-119 Rat Monoclonal Antibody [Clone ID: TER-119]

CAT#: AM31858PU-N

Ly-76 / TER-119 rat monoclonal antibody, clone TER-119, Purified

Product Images

Cell Source: Whole Blood

Specifications

Product Data

• Clone Name: TER-119
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry.
  This Antibody has been reported to work in Western Blot, Immunoprecipitation and Immunohistochemistry on Frozen and Paraffin Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Day 14 BALB/c fetal liver cells from Wistar Rat spleen.
• Specificity: Mouse Erythroid Cells (Ly-76).
  This anti-Mouse Erythroid cell (Ly-76) Monoclonal Antibody is selectively reactive with both fetal and adult erythroid cells.
  This monoclonal antibody (clone: TER119) is specific for cells at stages from early proerythroblast to mature erythrocytes.
  TER119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive with AM31858PU-N. TER119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers.
  This Monoclonal Antibody immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER119 in Western Blot analysis. It has been determined that the TER119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells.
• Formulation: PBS containing 0.02% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: Lymphocyte antigen 76, TER119
• Note: Test Results:
  Tissue Distribution by Flow Cytometry Analysis:
  Mouse Strain: ATH
  Cell Concentration: 1 x 10e6 cells per test
  Antibody Concentration Used: 0.5 ?g/10e6 cells
  Secondary Antibody Used: Goat Anti-Rat FITC
  
  Cell Source (Percentage of cells stained above control):
  Whole Bone Marrow (17.4%)
  Whole Blood (86.5%)
  *Blood was collected 1:1 in Alsever's and 0.1M Disodium EDTA was added 1:1 and incubated 10 minutes at room temperature followed by 3 washes with PBS.