TSG101 (201-281) Mouse Monoclonal Antibody [Clone ID: 5B7]

CAT#: AM31014PU-N

TSG101 (201-281) mouse monoclonal antibody, clone 5B7, Purified

Product Images

TSG101 monoclonal antibody clone 5B7 Western Blot analysis of TSG101 expression in K-562.

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  Detection limit for recombinant GST tagged TSG101 is approximately 0.03ng/ml as a capture antibody.

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  Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to TSG101 on HeLa cell. [antibody concentration 10 ug/ml]

Specifications

Product Data

• Clone Name: 5B7
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: TSG101 (NP_006283, 201 a.a. ~ 281 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to TSG101.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor susceptibility 101
• Database Link:
  Entrez Gene 7251 Human
  UniProt ID Q99816
• Background: The tumor susceptibility gene 101 (TSG101) was first discovered in a screen for potential tumor suppressors. Altered transcripts of this gene have been detected in sporadic breast carcinomas and other malignancies. In mice the gene has been shown to be essential for growth, proliferation and survival of mammary epithelial cells. TSG101 is also a component of the ESCRt-1 complex, a regulator of the vesicular trafficking process. In addition it is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies and is involved the budding of many viruses.
• Synonyms: TSG10; VPS23

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endocytosis