CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: EOS9.1]

CAT#: AM26792PU-N

CD95 (FAS) mouse monoclonal antibody, clone EOS9.1, Purified

Specifications

Product Data

• Clone Name: EOS9.1
• Applications: FC, FN
• Recommended Dilution: Flow cytometry.
  Functional application (in vitro induction of apoptosis).
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: P815 cells transfected with human CD95
• Specificity: This antibody recognizes CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
• Formulation: Tris buffered saline (TBS)
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM sodium azide, approx. pH 8.0
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: Fas cell surface death receptor
• Database Link:
  Entrez Gene 355 Human
  UniProt ID P25445
• Background: CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6