Amyloid beta (1-42 specific) Mouse Monoclonal Antibody [Clone ID: CA9 3B3]

CAT#: AM26393PU-L

Amyloid beta (1-42 specific) mouse monoclonal antibody, clone CA9 3B3, Aff - Purified

Product Images

Detection of A42 on 10 ng /dot of A40 or A42 using Clone CA9 3B3 at 1g/ml.

Specifications

Product Data

• Clone Name: CA9 3B3
• Applications: ELISA, WB
• Recommended Dilution: ELISA: In combination with capturer antiamyloid peptide N-terminal antibody (mAb clone NT 4A2, Cat.-No AM26042PU-N), the antibody can detect Aβ42 in Sandwich ELISA assay.
  Immunoblotting.
• Reactivities: Human, Mouse, Primate, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: KLH conjugated to a short peptide with amino acid sequence corresponding to the C-terminal of beta amyloid peptide 42.
• Specificity: This antibody recognizes the C-terminal sequence of beta amyloid peptide 42 and full length beta amyloid peptide 42.
  The antibody does not cross react with beta amyloid peptide 40 in dot blotting and ELISA. Cross-reactivity to beta amyloid peptide 43 is about 5% in ELISA.
• Formulation: 0.01M PBS pH7.2
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Amyloid beta peptide 42 (Aβ42) is best known for its role in the formation of senile plaques in the brain of patients with Alzheimer’s disease. Aβ42 and Aβ40 are the two major amyloid peptides that are produced after cleavage of amyloid precursor protein by secretases. Aβ42 (42 amino acids) is very fibrillogenic. The beta pleated structure of Aβ42 constituents the initial and key component of the insoluble amyloid fibril in senile plaque. It is widely accepted that Aβ42 contributes to the pathogenesis of Alzheimer’s disease. One proposition is that the deposition of amyloid fibril onto the brain tissue results in Alzheimer’s disease. Another is that the neurotoxicity of Aβ42 oligomer is the cause of the disease.