Jam3 Rat Monoclonal Antibody [Clone ID: JAM-C]

CAT#: AM26320PU-N

Jam3 rat monoclonal antibody, clone JAM-C, Purified

Specifications

Product Data

• Clone Name: JAM-C
• Applications: ELISA, FN, IHC, IP
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Flow cytometry: The typical starting working dilution is 1:50.
  Functional assays (inhibition of biological activity).
  Immunoassays (detector).
  Immunoprecipitation.
  
  The antibody cannot be used for Western blot and immunohistology on paraffin sections.
• Reactivities: Human, Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: The monoclonal antibody CRAM-18 F26 recognizes junctional adhesion molecule-C (JAM-C) also known as JAM-2, a 45 kD cell adhesion molecule (CAM).
  In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. Monoclonal antibody CRAM-18 F26 also reacts with human JAM-C. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
• Concentration: lot specific
• Purification: Protein G
• Conjugation: Unconjugated
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: junction adhesion molecule 3
• Database Link:
  Entrez Gene 83964 Mouse
  UniProt ID Q9D8B7
• Background: JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney.
• Synonyms: FLJ14529; JAM-2; JAM-3; JAM-C; JAMC