CD8A Mouse Monoclonal Antibody [Clone ID: 15-11C5]

CAT#: AM26177PU-N

CD8A mouse monoclonal antibody, clone 15-11C5, Purified

Specifications

Product Data

• Clone Name: 15-11C5
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry.
  Immunohistochemistry on Paraffin Sections.
  Immunohistochemistry on Frozen Sections.
  The typical starting working dilution is 1/10 for Flow Cytometry and 1/100 for immunohistology.
  
  
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: WAG/Rij spleen cells
• Specificity: The monoclonal antibody recognizes rat CD8 antigen (MW 34 kDa and 39 kDa) and is reactive with all common inbred strains. 15-11C5 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij spleen cells.
• Formulation: PBS with 50% (v/v) glycerol
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Preservative: 0.01% Sodium Azide
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8a molecule
• Database Link:
  UniProt ID P01732
• Background: CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8 alpha. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8 beta chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 alpha beta heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
• Synonyms: CD8 alpha chain, CD8A, MAL