ECs2973 Mouse Monoclonal Antibody [Clone ID: 13C4]

CAT#: AM26151PU-N

ECs2973 mouse monoclonal antibody, clone 13C4, Purified

Specifications

Product Data

• Clone Name: 13C4
• Applications: ELISA, FN, WB
• Recommended Dilution: Western blot: 1/10 as starting dilution.
  Fuctional Assay: Inhibition of the biological activity of the toxin.
• Reactivities: Escherichia coli
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The monoclonal antibody 13C4 recognizes the 1B subunit of Shiga-like toxin 1.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P69179
• Background: Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC.
  SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct.The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
• Synonyms: sltB, Verocytotoxin 1 subunit B, Verotoxin 1 subunit B