stxA2 Mouse Monoclonal Antibody [Clone ID: 11E10]

CAT#: AM26150PU-N

stxA2 mouse monoclonal antibody, clone 11E10, Purified

Specifications

Product Data

• Clone Name: 11E10
• Applications: ELISA, FN, WB
• Recommended Dilution: Western blot: 1:10 as starting dilution.
  Inhibition of the biological activity of the toxin.
• Reactivities: Escherichia coli
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes the 2A subunit of Shiga-like toxin 2.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Product should be stored at 2-8 °C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P09385
• Background: Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11, and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC.
  SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. Antibodies to SLT-II can also neutralize a variant of SLT-II (designated SLT-Iiv) produced by strains of E.coli that cause edema. SLT-IIv is cytotoxic for Vero but not HeLa cells, distinguishing it from SLT-II.
  The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
• Synonyms: SLT-2 A subunit, SLT-2a, SLT-IIa, stx2A, stxA2, Verotoxin 2 subunit A, Verocytotoxin 2 subunit A