BPV-1 Protein E2 (310-410) Mouse Monoclonal Antibody [Clone ID: 5H4]

CAT#: AM26106PU-N

BPV-1 Protein E2 (310-410) mouse monoclonal antibody, clone 5H4, Aff - Purified

Specifications

Product Data

• Clone Name: 5H4
• Applications: ELISA, FN, IF
• Recommended Dilution: Immunoflourescence.
  ELISA.
  Functional application: specifically dissociates E2 protein from DNA (Kurg et al, 1999).
  This is a conformational antibody and does not work in Western immunoblotting analysis or in other denaturative conditions.
• Reactivities: Bovine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody detects Bovine Papillomavirus E2-protein, mapped to amino acids 310-410.
• Formulation: PBS with 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20 to -70 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P03122
• Background: The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
• Synonyms: BPV1 Regulatory protein E2