TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 2C8]

CAT#: AM26058PU-N

TNF alpha (TNF) mouse monoclonal antibody, clone 2C8, Aff - Purified

Specifications

Product Data

• Clone Name: 2C8
• Applications: ELISA, FN
• Recommended Dilution: This Monoclonal antibody 2C8 is useful for studying biological effects of TNF-alpha in vitro and in vivo.
  The antibody is Neutralizing TNF-alpha effects in vitro.
  2C8 can also be used as a Capture antibody in a Sandwich ELISA to quantitate TNF-alpha.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human TNF alpha
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore with 0.5ml distilled water.
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography, Endotoxin depleted
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Background:

  Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms.
  The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines.
  Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways.
  The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.

• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus