ERK1 / ERK2 Mouse Monoclonal Antibody [Clone ID: IL-13]

CAT#: AM20636PU-N

ERK1 / ERK2 mouse monoclonal antibody, clone IL-13, Purified

Specifications

Product Data

• Clone Name: IL-13
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 0.25 - 0.5 μg/ml.
  Immunohistochemistry on paraffin sections: 0.4 - 1 μg/ml.
  Immunocytochemistry.
• Reactivities: Human, Insect, Mouse, Rat, Yeast
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody reacts to ERK1 / ERK2.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: 0,1 mg/ml (after reconstitution with PBS)
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.
• Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK