PAK4 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C1]

CAT#: AM20217PU-N

PAK4 (incl. pos. control) mouse monoclonal antibody, clone 6C1, Purified

Product Images

Detection of endogenous PAK4: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20217PU-N PAK4 antibody (Clone 6C1) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D

Specifications

Product Data

• Clone Name: 6C1
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for more details).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to hemocyanin
  Epitope: Peptide derived from the kinase activation loop.
• Specificity: Recognizes p21 activated kinase 4 (PAK4).
• Formulation: 1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Conjugation: Unconjugated
• Storage: For long-term storage, freeze lyophilizate upon arrival (-20°C).
  Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year.
  Avoid repeated freeze / thaw cycles.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
• Gene Name: p21 (RAC1) activated kinase 4
• Database Link:
  Entrez Gene 70584 MouseEntrez Gene 292756 RatEntrez Gene 10298 Human
  UniProt ID O96013
• Background: p21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.
• Synonyms: PAK 4, PAK-4, p21-activated kinase 4, KIAA1142
• Note: Mol. weight: 60 kDa
  
  Protocol: Positive Control Provided.
  Cell lysate from untreated SW480 cells
  
  Description: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)
  
  Format: Lyophilized cell lysate from serum starved SW480 cells.
  
  Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway