CD28 Mouse Monoclonal Antibody [Clone ID: CD28.2]

CAT#: AM12006PU-N

CD28 mouse monoclonal antibody, clone CD28.2, Purified

Product Images

Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) purified.

Specifications

Product Data

• Clone Name: CD28.2
• Applications: FC, FN, IF, IHC, IP, WB
• Recommended Dilution: Flow cytometry.
  Immunoprecipitation.
  Western blot.
  Immunohistochemistry (frozen sections).
  Immunocytochemistry.
  Functional application: T cell costimulation.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
• Specificity: This antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A affinity chromatography; purity > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD28 molecule
• Database Link:
  Entrez Gene 940 Human
  UniProt ID P10747
• Background: CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
• Synonyms: TP44

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, T cell receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis