SRC Mouse Monoclonal Antibody [Clone ID: 17AT28]

CAT#: AM11074PU-N

SRC mouse monoclonal antibody, clone 17AT28, Purified

Product Images

Figure 1. Western blot analysis using anti-Src Mab to detect Src in Jurkat cell lysate.

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  Figure 2. FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4°C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.

Specifications

Product Data

• Clone Name: 17AT28
• Applications: IHC, IP, WB
• Recommended Dilution: ELISA: 1/1,000
  Western blotting: 1/100-1/500
  Immunohistochemistry: 1/50-1/100
  Immunoprecipitation: 2 ug/test.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: This antibody is generated from mouse immunized with a recombinant protein of full length human SRC.
• Specificity: This antibody is specific to Src.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: SRC proto-oncogene, non-receptor tyrosine kinase
• Database Link:
  Entrez Gene 6714 Human
  UniProt ID P12931
• Background: The gene for SRC is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. SRC, the protein encoded by this gene, is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer.
• Synonyms: c-Src, pp60c-src