Oct4 (POU5F1) Mouse Monoclonal Antibody [Clone ID: C-10]

CAT#: AM10125SU-N

Oct4 (POU5F1) mouse monoclonal antibody, clone C-10, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Seminoma stained with OCT-4 antibody Cat.-No. AM10125SU-N using peroxidase conjugate and DAB chromogen. Note the nuclear staining of seminoma cells.

Specifications

Product Data

• Clone Name: C-10
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Seminoma.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Amino acids 1-134 of Oct-4 of Human origin.
• Specificity: Oct-3/4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of Mouse, Rat and Human origin by Western Blotting, Immunoprecipitation, Immunofluorescence, and Immunohistochemistry on Paraffin.
  Cellular Localization: Nuclear.
• Formulation: State: Purified
  State: Tris-HCl buffer containing stabilizing protein and 0.09 % sodium azide.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: POU class 5 homeobox 1
• Database Link:
  Entrez Gene 18039 MouseEntrez Gene 83613 RatEntrez Gene 5460 Human
  UniProt ID Q01860
• Background: Transcription factors containing the POU homeo domain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct-3/4 is required to sustain stem-cell self renewal, and up or down regulation induce divergent developmental programmes. Two isoforms of Oct-3, termed Oct-3A and Oct-3B, are generated by alternative splicing. The gene which encodes Oct-3/4 maps to human chromosome 6p21.3.
• Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding transcription factor 3, Oct-3, Oct-4

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)