CREB1 pSer133 Mouse Monoclonal Antibody [Clone ID: A3A4]

CAT#: AM09297PU-N

CREB1 pSer133 mouse monoclonal antibody, clone A3A4, Aff - Purified

Specifications

Product Data

• Clone Name: A3A4
• Applications: ELISA
• Recommended Dilution: Indirect ELISA: The antibody is reactive to serine133 phosphorylated Human CREB peptide and reactive to Serine133 phosphorylated human CREB peptide conjugated with KLH or BSA.
  The suggested antigen coating quantity is 1 µg/ml of peptide/or peptide conjugating in pH 9.5 carbonate buffer, 100 µl/well.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide of a phosphorylated fragment of CREB [pSer133], conjugated with KLH
• Specificity: Reactive to Serine133 phosphorylated Human CREB peptide fragment, and reactive to KLH or BSA conjugated Serine133 phosphorylated Human CREB peptide fragment.
• Formulation: 0.01 M PBS, pH 7.0 without preservatives
  State: Aff - Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cAMP responsive element binding protein 1
• Database Link:
  Entrez Gene 1385 Human
  UniProt ID P16220
• Background: Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.
• Synonyms: CREB-1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer