CRABP2 Mouse Monoclonal Antibody [Clone ID: AT2E11]

CAT#: AM09062PU-S

CRABP2 mouse monoclonal antibody, clone AT2E11, Purified

Product Images

Western blot analysis: Cell lysates of mouse eye (60 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP2 antibody (1/250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: -N CRABP2 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Uterus at 10 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: AT2E11
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/250-1/1,000).
  Immunohistochemistry on Paraffin Sections (10 µg/ml).
  This CRABP2 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) Human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human CRABP2 (1-138aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse CRABP2. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cellular retinoic acid binding protein 2
• Database Link:
  Entrez Gene 12904 MouseEntrez Gene 1382 Human
  UniProt ID P29373
• Background: The cellular retinoic acid-binding protein II (CRABP-II) is involved in the conversion of vitamin A into its intracellular active form retinoic acid, which regulate the genes responsible for lipid metabolism and adipocyte differentiation. CRABP2 gene is located on chromosome 1q21-23 and this region has been linked with related disorders such as familial combined hyperlipidemia (FCHL) and type 2 diabetes mellitus.
• Synonyms: CRABP-II

Reference Data

• Protein Families: Druggable Genome, Transcription Factors