14-3-3 theta (YWHAQ) (1-245) Mouse Monoclonal Antibody [Clone ID: AT1A1]

CAT#: AM09060PU-N

14-3-3 theta (YWHAQ) (1-245) mouse monoclonal antibody, clone AT1A1, Purified

Product Images

Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: 14-3-3 tau antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex.

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  Immunohistochemistry: 14-3-3 tau antibody staining of Formalin-Fixed, Paraffin-Embedded Human Pancreas.

Specifications

Product Data

• Clone Name: AT1A1
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/2,000).
  Immunohistochemistry on Paraffin Sections (10 µg/ml).
  This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  Flow cytometry.
  Immunofluorescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human 14-3-3 tau (aa 1-245) purified from E. coli
• Specificity: The antibody recognizes Human 14-3-3 protein theta (tau) at aa 1-245.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
• Database Link:
  Entrez Gene 10971 Human
  UniProt ID P27348
• Background: The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.
• Synonyms: 14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection