FLIP (CFLAR) (1-376) Mouse Monoclonal Antibody [Clone ID: AT8B12]

CAT#: AM09055PU-N

FLIP (CFLAR) (1-376) mouse monoclonal antibody, clone AT8B12, Purified

Product Images

Western blot analysis: Cell lysates of mouse kidney, MCF7 and rat spleen (40 ug) were resolved by SDS-PAGE, trans-ferred to NC membrane and probed with FLIP antibody. (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT8B12
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/500-1/1000.
  Immunofluorescence.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human FLIP (aa 1-376) purified from E. coli.
• Specificity: The antibody recognizes FLIP.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CASP8 and FADD like apoptosis regulator
• Database Link:
  Entrez Gene 12633 MouseEntrez Gene 117279 RatEntrez Gene 8837 Human
  UniProt ID O15519
• Background: FLIP, also known as CASP8 and FADD-like apoptosis regulator (CFLAR), contains two death effector domains (DEDs) and a caspase-like domain. FLIP may play a crucial role between cell survival and cell death pathway in mammalian cells and interacts with adapter protein FADD and Caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (Death Receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (Tumor Necrosis Factor Receptor 1).
• Synonyms: c-FLIP, CLARP, MRIT, CASH, FLAME-1, CASP8AP1, Usurpin

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis