AVEN Mouse Monoclonal Antibody [Clone ID: 3G4]

CAT#: AM09049PU-S

AVEN mouse monoclonal antibody, clone 3G4, Purified

Product Images

The extracts of mouse kidney (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: The extracts of mouse kidney (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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  Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 3G4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1:1,000 - 1:3,000).
  Immunohistochemistry on paraffin sections (1:50 - 1:100).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human PDCD12 (aa 254-362) purified from E. coli
• Specificity: The antibody recognizes AVEN / PDCD12.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: apoptosis and caspase activation inhibitor
• Database Link:
  Entrez Gene 57099 Human
  UniProt ID Q9NQS1
• Background: PDCD12 (Programmed cell death 12), also referred to as AVEN, represents a new class of cell death regulator. PDCD12 is a conserved intracellular membrane protein that blocks apoptosis impeded by Apaf-1 mediated caspase activation. Bcl-2 and BAD also interact with PDCD12 to prevent apoptosis. It is expressed in a wide variety of normal tissues including heart, skeletal muscle, kidney, liver, testis, and diseases such as cancer.
• Synonyms: PDCD12