IL33 Mouse Monoclonal Antibody [Clone ID: 4E9]

CAT#: AM09020PU-N

IL33 mouse monoclonal antibody, clone 4E9, Purified

Product Images

The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis on Jurkat cells: The Cell lysates of Jurkat (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 4E9
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:2,000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human IL-33 (112-270 aa) purified from E. coli
• Specificity: The antibody recognizes human IL-33.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 33
• Database Link:
  Entrez Gene 90865 Human
  UniProt ID O95760
• Background: Interleukin 33 (IL-33) is a 32 kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T-cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12 kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
• Synonyms: IL-33, IL1F11, IL-1F11, NFHEV, NF-HEV, C9orf26