Heat Shock Protein 90 / HSP90 (alpha + beta) Mouse Monoclonal Antibody [Clone ID: 4F10]

CAT#: AM09018PU-S

Heat Shock Protein 90 / HSP90 (alpha + beta) mouse monoclonal antibody, clone 4F10, Purified

Product Images

Cell lysates of HeLa (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of HeLa (each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL det

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  Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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  Immunohistochemistry: Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 4F10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1:1,000 - 1: 2,000).
  Immunohistochemistry (1:100 - 1:200).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human Hsp90 (1-732 aa) purified from E.coli
• Specificity: The antibody recognizes human Hsp90.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: heat shock protein 90kDa alpha family class A member 1
• Database Link:
  Entrez Gene 3320 Human
  UniProt ID P07900
• Background: Hsp90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.
• Synonyms: HSP90AB1, HSP90B, HSPC2, HSPCB, HSP84, HSP-90, HSP-84, Heat shock protein HSP 90-beta, HSP84, HSP-84, HSP90AA1, HSP90A, HSPC1, HSPCA, HSP86, HSP-86, Renal carcinoma antigen NY-REN-38, Heat shock protein HSP 90-alpha, HSP86, HSP-86

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer