liver FABP (FABP1) Mouse Monoclonal Antibody [Clone ID: 2G4]

CAT#: AM09011PU-N

liver FABP (FABP1) mouse monoclonal antibody, clone 2G4, Purified

Product Images

Western blot analysis on HepG2 cells: Cell lysates of HepG2 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human FABP1 (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: FABP1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: FABP1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Liver at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: FABP1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon Cancer Tissue at 1/1000 for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 2G4
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/1,000-1/2,000.
  Flow Cytometry.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 1/100-1/300.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human FABP1 (1-127 aa) purified from E. coli.
• Specificity: The antibody recognizes Human FABP1.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: fatty acid binding protein 1
• Database Link:
  Entrez Gene 2168 Human
  UniProt ID P07148
• Background: FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallel β strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism.
• Synonyms: FABPL, L-FABP, Liver-type fatty acid-binding protein

Reference Data

• Protein Pathways: PPAR signaling pathway