Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: k2D5]

CAT#: AM09002PU-S

Integrin beta 1 (ITGB1) mouse monoclonal antibody, clone k2D5, Purified

Product Images

Cell lysates of U937 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD29 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis on U937 cells: Cell lysates of U937 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human CD29 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: -N CD29 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Immunohistochemistry: -N CD29 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: k2D5
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/500-1/1,000.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human CD29 (34-141 aa) purified from E. coli
• Specificity: The antibody recognizes Human CD29.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit beta 1
• Database Link:
  Entrez Gene 3688 Human
  UniProt ID P05556
• Background: CD29, also known as Integrin beta 1, is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. Interaction between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
• Synonyms: Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12