Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: J4H9]

CAT#: AM09000PU-S

Amyloid Precursor Protein (APP) mouse monoclonal antibody, clone J4H9, Purified

Product Images

Western blot analysis on Mouse brain: Tissue lysates of mouse brain (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human APP antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry: -N APP antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex.

Specifications

Product Data

• Clone Name: J4H9
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1/1,000-1/2,000).
  Immunohistochemistry on Paraffin Sections (2.5 µg/ml).
  This APP antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human APP (18-289aa) purified from E. coli
• Specificity: The antibody recognizes APP.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: amyloid beta precursor protein
• Database Link:
  Entrez Gene 351 Human
  UniProt ID P05067
• Background: Amyloid precursor protein (APP) is the precursor molecule whose proteolysis generates amyloid beta (Aβ), a 39- to 42- amino acid peptide and this amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer’s diseases patients. APP is an integral membrane protein that is phosphorylated in the cytoplasmic and extracellular domains. It has been reported that cell-surface APP plays a role in neurite extension of primary cultured hippocampal neurons. The large extracellular domain of APP is also reported to bind extracellular matrix molecules such as heparin, laminin, and collagen, which can mediate cell adhesion and neurite outgrowth. Abnormal regulation of the metabolism of APP may contribute to the deposition of plaques.
• Synonyms: Alzheimer disease amyloid protein, Amyloid Precursor Protein, ABPP, APPI, PreA4, Cerebral vascular amyloid peptide, CVAP