Cytokeratin 18 (KRT18) (N-term) Mouse Monoclonal Antibody [Clone ID: SB38b]

CAT#: AM08175PU-N

Cytokeratin 18 (KRT18) (N-term) mouse monoclonal antibody, clone SB38b, Purified

Specifications

Product Data

• Clone Name: SB38b
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/5,000-1/20,000.
  Western Blot: 1/2,000-1/4,000.
  Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections): 10 µg/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant N-terminal fragment of keratin 18.
• Specificity: This antibody reacts specifically specifically with Keratin 18 and recombinant N-terminal Keratin 18.
• Formulation: 100 mM Borate buffered saline, pH 8.0 without preservatives or amine-containing buffer salts.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 18
• Database Link:
  Entrez Gene 3875 Human
  UniProt ID P05783
• Background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. (Ref.1-6) Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein of approximately 48 kDa. It exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia, and is often co-expressed with cytokeratin 8. (Ref.6-8) Tissues from the gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
• Note: Chatacterization: To ensure acceptable performance, each batch of product is tested by ELISA, Immunohistochemical staining (HT29 and HCC38 cells) and Western blotting (HT29 and HCC38 cell lysates) to conform to characteristics of a standard reference reagent.