CD3E Mouse Monoclonal Antibody [Clone ID: BB23-8E6]

CAT#: AM08105BT-N

CD3E mouse monoclonal antibody, clone BB23-8E6, Biotin

Product Images

Immunofluorescent Staining: Peripheral blood mononuclear cells were isolated from heparinized porcine blood on Ficoll-Hypaque density gradients and incubated with Mouse anti-Porcine CD3e-Biotin, then detected with Streptavidin-FITC. Lymphocytes were gated using light scatter criteria and analyzed on a FACScan (TM) flow cytometer (BD, San Jose, CA). Amount Used: 0.3 ug/10e6 cells.

Specifications

Product Data

• Clone Name: BB23-8E6
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This antibody recognizes Porcine CD3e (Mr. 20 kDa).
  The Monoclonal antibody BB238E6 is Mitogenic when presented to peripheral blood mononuclear cells in immobilized form. (Ref.1)
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: Biotin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q7YRN2
• Background: CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains.
• Synonyms: T3/Leu-4