Eng Rat Monoclonal Antibody [Clone ID: MJ7/18]

CAT#: AM08073PU-N

Eng rat monoclonal antibody, clone MJ7/18, Purified

Specifications

Product Data

• Clone Name: MJ7/18
• Applications: FC, IHC, WB
• Recommended Dilution: Flow Cytometry: Identification and enumeration of CD105+ cells. (< / = 1 µg/10e6 cells). (Ref.1)
  Immunohistochemistry on Frozen Sections.
  Western Blotting. (Ref.1)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Inflamed mouse skin. (Ref.1)
• Specificity: This antibody recognises Mouse Endoglin/CD105 (Mr. 90kDa)
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: endoglin
• Database Link:
  Entrez Gene 13805 Mouse
  UniProt ID Q63961
• Background: CD105/endoglin is a disulfide-linked type II transmembrane homodimer that is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells. (Ref.2) Both mouse and human CD105 exhibit > 70% sequence homology in their cytoplasmic domains with type III Transforming Growth Factor beta (TGF-beta) receptor. (Ref.3) CD105 has been shown to be one of several receptors for the various isoforms of TGF-beta, which in turn is one of a family of proteins involved in regulation of cell differentiation, migration of cells and control of the immune response. (Ref.4)
• Synonyms: ENG, END, HHT1, ORW, ORW1