Thy1 Rat Monoclonal Antibody [Clone ID: 30-H12]

CAT#: AM08055FC-S

Thy1 rat monoclonal antibody, clone 30-H12, FITC

Product Images

Immunofluorescent Staining: BALB/c splenocytes were double-stained with Rat anti-Mouse CD90.2-FITC and Rat anti-Mouse CD19-PE. Small lymphocytes were then gated and analyzed on a FACScan (TM) flowcytometer (BDIS, San Jose, CA). Amount Used: 0.3 ug/10e6 cells.

Specifications

Product Data

• Clone Name: 30-H12
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.4,5)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This antibody recognises CD90.2/Thy-1.2 alloantigen (Mr. 18 kDa).
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: thymus cell antigen 1, theta
• Database Link:
  Entrez Gene 21838 Mouse
  UniProt ID P01831
• Background: CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (Ref.1-3)
  The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with Mouse and Rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J,PL). (Ref.2-5).
• Synonyms: Thy1.2, Thy-1.2, CD90, Thy-1