Cd86 Rat Monoclonal Antibody [Clone ID: GL1]

CAT#: AM08053LE-N

Cd86 rat monoclonal antibody, clone GL1, Low Endotoxin

Specifications

Product Data

• Clone Name: GL1
• Applications: FC
• Recommended Dilution: Flow Cytometry. (Ref.1-7)
  Immunoprecipitation. (Ref.1)
  Immunohistochemistry Frozen Sections. (Ref.6)
  Functional Assays: In vivo and In vitro blocking assays. (Ref.1,3,5)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: LPS-activated mouse B cells.(Ref.1)
• Specificity: This antibody recognises CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4.
  The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells. (Ref.1,3,5)
• Formulation: PBS without preservatives.
  State: Low Endotoxin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD86 antigen
• Database Link:
  Entrez Gene 12524 Mouse
  UniProt ID P42082
• Background: CD86 (also known as B7-2) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. (Ref.1-5) CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. (Ref.1,5) CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. (Ref.1-9).
• Synonyms: CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1