CD49e / ITGA5 Rat Monoclonal Antibody [Clone ID: 5H10-27]

CAT#: AM08047LE-N

CD49e / ITGA5 rat monoclonal antibody, clone 5H10-27, Low Endotoxin

Specifications

Product Data

• Clone Name: 5H10-27
• Applications: FC, FN, IHC
• Recommended Dilution: Flow Cytometry. (Ref.1)
  Immunohistochemistry on Frozen Sections: (Ref.7)
  Functional Assays:
  In vitro blocking of adhesion. (Ref.1,2-4,6)
  T-cell co-stimulation. (Ref.3)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C57BL/6 x A/J F1 mouse mast cell line MC/9. (Ref.1)
• Specificity: This antibody is specific to alpha-5 subunit of the integrin alpha-5 beta-1 Fibronectin Receptor.
  It reacts with the alpha-5 chain (VLA-5) of the CD49e/CD29 heterodimeric Fibronectin Receptor. (Ref.1-5)
  Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. (Ref.1,2-4,6) Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody. (Ref.3)
• Formulation: PBS containing no preservatives.
  State: Low Endotoxin
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin alpha 5 (fibronectin receptor alpha)
• Database Link:
  Entrez Gene 16402 Mouse
  UniProt ID P11688
• Background: Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines.
• Synonyms: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA