Cd3e Rat Monoclonal Antibody [Clone ID: C363.29B (YCD3-1)]

CAT#: AM08026FC-S

Cd3e rat monoclonal antibody, clone C363.29B (YCD3-1), FITC

Product Images

Immunofluorescent Staining: Cells from BALB/c mesenteric lymph nodes were double-stained with anti-Mouse CD3e-FITC and anti-Mouse CD45R/B220-R-PE (clone RA3-6B2), gated on small lymphocytes and analyzed using a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount used: < / = 3 ug/10e6.

Specifications

Product Data

• Clone Name: C363.29B (YCD3-1)
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: Identification and enumeration of CD3+ (≤ 3 µg/10⁶ cells) (See also Ref.1,2).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: IL-4 producing Th2 cell lines including D10
• Specificity: This antibody is specific to CD3e (Mr. 25 kD).
• Formulation: PBS containing 0.09% Sodium Azide as preservative
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD3 antigen, epsilon polypeptide
• Database Link:
  Entrez Gene 12501 Mouse
  UniProt ID P22646
• Background: CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2)
  CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
  The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4)
  The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)
• Synonyms: T3/Leu-4