ITK Mouse Monoclonal Antibody [Clone ID: 5G6]

CAT#: AM06655SU-N

ITK mouse monoclonal antibody, clone 5G6, Ascites

Product Images

Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)

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  Western blot analysis using ITK mouse mAb against Jurkat cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

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  Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).

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Specifications

Product Data

• Clone Name: 5G6
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human ITK expressed in E. Coli.
  
• Specificity: This antibody reacts to ITK.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 72 kDa
• Gene Name: IL2 inducible T-cell kinase
• Database Link:
  Entrez Gene 3702 Human
  UniProt ID Q08881
• Background: This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
• Synonyms: EMT, LYK