RICTOR Mouse Monoclonal Antibody [Clone ID: 4H5]

CAT#: AM06502SU-N

RICTOR mouse monoclonal antibody, clone 4H5, Ascites

Product Images

Western blot analysis using RICTOR antibody Cat.-No AM06502SU-N against Hela (1), PANC-1 (2), MOLT4 (3), and HepG2 (4) cell lysate.

Specifications

Product Data

• Clone Name: 4H5
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human RICTOR expressed in E. Coli.
• Specificity: Recognizes RICTOR
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 192 kDa
• Gene Name: RPTOR independent companion of MTOR complex 2
• Database Link:
  Entrez Gene 253260 Human
  UniProt ID Q6R327
• Background: Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals . mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1 and rictor . The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro . This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes . This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR .
• Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3