RAG2 Mouse Monoclonal Antibody [Clone ID: 4D5]

CAT#: AM06429SU-N

RAG2 mouse monoclonal antibody, clone 4D5, Ascites

Product Images

Western blot analysis using RAG2 mouse mAb against RAG2 (AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate.

Specifications

Product Data

• Clone Name: 4D5
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human RAG2(350-527aa) expressed in E. Coli.
• Specificity: This antibody reacts to RAG2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
  
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 59 kDa
• Gene Name: recombination activating gene 2
• Database Link:
  Entrez Gene 5897 Human
  UniProt ID P55895
• Background: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
• Synonyms: RAG-2