alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: 6E6]

CAT#: AM06377PU-N

alpha 1 Fetoprotein (AFP) mouse monoclonal antibody, clone 6E6, Aff - Purified

Product Images

Western blot analysis using AFP mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.

Specifications

Product Data

• Clone Name: 6E6
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human AFP expressed in E. Coli.
• Specificity: This antibody reacts to AFP.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 50% glycerol
  Preservative: 0,03% sodium azide
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 69 kDa
• Gene Name: alpha fetoprotein
• Database Link:
  Entrez Gene 174 Human
  UniProt ID P02771
• Background: AFP (alpha-fetoprotein), a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
• Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP