PTEN Mouse Monoclonal Antibody [Clone ID: 1B8]

CAT#: AM06375PU-N

PTEN mouse monoclonal antibody, clone 1B8, Aff - Purified

Product Images

Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

• 

  Western blot analysis using PTEN mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.

• 

  Flow cytometric analysis of Hela cells using PTEN mouse mAb (right) and negative control (left).

Specifications

Product Data

• Clone Name: 1B8
• Applications: ELISA, IF, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of PTEN expressed in E. Coli.
• Specificity: This antibody reacts to PTEN.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 50% glycerol
  Preservative: 0,03% sodium azide
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 54 kDa
• Gene Name: phosphatase and tensin homolog
• Database Link:
  Entrez Gene 5728 Human
  UniProt ID P60484
• Background: PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
• Synonyms: MMAC1, TEP1