Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: 4A1]

CAT#: AM06345SU-N

Ki67 (MKI67) mouse monoclonal antibody, clone 4A1, Ascites

Product Images

Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D), showing nuclear localization using KI67 antibody Cat.-No AM06345SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 4A1
• Applications: IHC
• Recommended Dilution: ELISA: 1/10000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.
• Specificity: Recognizes Ki67, also known as MKI67
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 358 kDa
• Gene Name: marker of proliferation Ki-67
• Database Link:
  Entrez Gene 4288 Human
  UniProt ID P46013
• Background: Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
• Synonyms: Ki67 antigen, MKI67, Proliferation marker