Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: 6F5]

CAT#: AM06340SU-N

Superoxide Dismutase 1 (SOD1) mouse monoclonal antibody, clone 6F5, Ascites

Product Images

Western blot analysis using SOD1 antibody Cat.-No AM06340SU-N against Hela (LAne 1), NIH/3T3 (LAne 2), A549 (Lane 3) and A431 (Lane 4) cell lysate.

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  Confocal immunofluorescence analysis of 3T3-L1 cells using SOD1 antibody Cat.-No AM06340SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

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  Confocal Immunofluorescence analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 antibody Cat.-No AM06340SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 6F5
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1/10,000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunocytochemistry: 1/200 - 1/1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human SOD1 expressed in E. Coli.
• Specificity: Recognizes SOD1 (superoxide dismutase 1, soluble).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 18 kDa
• Gene Name: superoxide dismutase 1, soluble
• Database Link:
  Entrez Gene 6647 Human
  UniProt ID P00441
• Background: SOD1 (superoxide dismutase 1, soluble), also known as ALS. The protein binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons. Rare transcript variants have been reported for this gene.
• Synonyms: SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1