WNT5A Mouse Monoclonal Antibody [Clone ID: 3D10]

CAT#: AM06331SU-N

WNT5A mouse monoclonal antibody, clone 3D10, Ascites

Product Images

Western blot analysis using WNT5A antibody Cat.-No AM06331SU-N against HEK293 (Lane 1) and WNT5A-hIgGFc transfected HEK293 cell lysate (Lane 2).

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  Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A antibody Cat.-No AM06331SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 3D10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of WNT5A expressed in E. Coli.
• Specificity: Recognizes Wingless-type MMTV integration site family (WNT5A).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42.3 kDa
• Gene Name: Wnt family member 5A
• Database Link:
  Entrez Gene 7474 Human
  UniProt ID P41221
• Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
• Synonyms: Protein Wnt-5a