Eph receptor B4 (EPHB4) Mouse Monoclonal Antibody [Clone ID: 5B5]

CAT#: AM06328SU-N

Eph receptor B4 (EPHB4) mouse monoclonal antibody, clone 5B5, Ascites

Product Images

Western blot analysis using EphB4 antibody Cat.-No AM06328SU-N against extracellular domain of human EphB4 (aa16-539).

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  Confocal Immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using EphB4 antibody Cat.-No AM06328SU-N (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 5B5
• Applications: IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line.
• Specificity: Recognizes EPH receptor B4 (EphB4).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 108 kDa
• Gene Name: EPH receptor B4
• Database Link:
  Entrez Gene 2050 Human
  UniProt ID P54760
• Background: EPH receptor B4 (EphB4), also known as Htk, Myk1, Tyro11 is a member of the Eph receptor family, which bind the ephrin ligand family. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The EphB4 protein binds to ephrin-B2 and plays an essential role in vascular development.
• Synonyms: Ephrin type-B receptor 4, HTK, TYRO11