Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: 1C12B8]

CAT#: AM06242SU-N

Chk2 (CHEK2) mouse monoclonal antibody, clone 1C12B8, Ascites

Product Images

Western blot analysis using CHK2 antibody Cat.-No AM06242SU-N against cell lysate from various cell types.

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  Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 antibody Cat.-No AM06242SU-N

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  Confocal immunofluorescence analysis of Hela cells using CHK2 antibody Cat.-No AM06242SU-N (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.

Specifications

Product Data

• Clone Name: 1C12B8
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blotting: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.
• Specificity: Recognizes CHK2
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: checkpoint kinase 2
• Database Link:
  Entrez Gene 11200 Human
  UniProt ID O96017
• Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
• Synonyms: CHEK2, CHEK-2, CHK-2, RAD53, Cds1