RET Mouse Monoclonal Antibody [Clone ID: 8D10C9]

CAT#: AM06194SU-N

RET mouse monoclonal antibody, clone 8D10C9, Ascites

Product Images

Western blot analysis using RET antibody Cat.-No AM06194SU-N against truncated RET recombinant protein (Lane 1) and RET (aa 658-1063)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).

Specifications

Product Data

• Clone Name: 8D10C9
• Applications: WB
• Recommended Dilution: ELISA: 1/10000.
  Western Bloting: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of RET (aa896-1063) expressed in E. Coli.
• Specificity: Recognizes RET (ret proto-oncogene).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: ret proto-oncogene
• Database Link:
  Entrez Gene 5979 Human
  UniProt ID P07949
• Background: RET (ret proto-oncogene) is a member of the cadherin superfamily and a receptor tyrosine kinase, which are cell-surface molecules that transduce signals for cell growth and differentiation. It can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.
• Synonyms: C-ret, CDHF12